Acrosin, Proacrosin, and Acrosin Inhibitor of Guinea Pig Spermatozoa Capacitated and Acrosome-Reacted in vitro
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چکیده
منابع مشابه
Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen.
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acro...
متن کاملImmunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa.
Mammalian sperm acrosomes contain a trypsin-like protease called acrosin which causes limited and specific hydrolysis of the extracellular matrix of the mammalian egg, the zona pellucida. Acrosin was localized on hamster, guinea-pig and human sperm using monoclonal and polyclonal antibodies to human acrosin labelled with colloidal gold. This was visualized directly with transmission electron mi...
متن کاملThe histochemical localization of acrosin in guinea-pig sperm after the acrosome reaction.
The protease acrosin is widely considered to be an essential component of a zona lysin which enables sperm to penetrate the zona pellucida of the egg. Sperm form a characteristic penetration slit little wider than the sperm head itself and this has long suggested that any zona lysin is attached to the sperm surface after an acrosome reaction. This paper provides the first ultrastructural eviden...
متن کاملStudies on acrosome labelling of mammalian spermatozoa by radioactive sugars.
The localization of glycoprotein synthesis and storage was studied during acrosome formation in guinea-pig using fine-structure autoradiography after (3H)-fucose incorporation. Three days after (3H)-fucose injection, labelling in spermatids was concentrated in the matrix of developing acrosomes, and it was evident that the fucosylation of acrosomal glycoproteins largely overshadowed the fucosyl...
متن کاملIdentification of zona- and fucoidan-binding proteins in guinea-pig spermatozoa and mechanism of recognition.
Binding of guinea-pig spermatozoa to the zona pellucida of homologous eggs has been reported to involve 'receptors' on the inner acrosomal membrane (Huang et al. 1981). These receptors can be blocked by sulphated polysaccharides such as fucoidan (Huang and Yanagimachi, 1984). The aims of the present investigation were to identify these putative zona receptors using 125I-fucoidan as a probe and ...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1981
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod25.1.44